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Analytical Technologies for Liquid Biopsy of Subcellular Materials
Camila D M Campos1,2, Katie Childers3,4, Sachindra S T Gamage4,5
1Life Science Department, Imec, 3001 Leuven, Belgium.
Summary
Subcellular liquid biopsy markers from blood offer disease insights but require enrichment. This article reviews analytical platforms for isolating these vital markers, evaluating their performance for improved diagnostics.
Area of Science:
- Biomarker Discovery
- Molecular Diagnostics
- Analytical Chemistry
Background:
- Liquid biopsies provide non-invasive disease monitoring via circulating biomarkers.
- Subcellular components like cell-free DNA, microRNA, and extracellular vesicles are key liquid biopsy analytes.
- Enrichment is crucial as these markers are scarce and susceptible to sample matrix interference.
Purpose of the Study:
- To review current and emerging analytical platforms for isolating subcellular liquid biopsy markers.
- To evaluate the performance metrics of these enrichment platforms.
Main Methods:
- Literature review of analytical enrichment techniques for subcellular liquid biopsy markers.
- Comparative analysis of platform figures of merit: recovery, throughput, and purity.
Main Results:
- Various enrichment platforms exist, each with distinct advantages and limitations.
- Key performance indicators (recovery, throughput, purity) vary significantly across platforms.
- Ongoing development aims to enhance sensitivity and specificity of marker isolation.
Conclusions:
- Effective enrichment of subcellular liquid biopsy markers is essential for accurate molecular profiling.
- Platform selection depends on specific clinical application and required analytical performance.
- Advancements in enrichment technologies will improve the utility of liquid biopsies in disease management.
