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Databases for technical aspects of immunohistochemistry: 2021 update.

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  • 1Conference on Experimental Animal Histopathology.

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Summary

This report details technical immunohistochemistry (IHC) aspects and antibody selection for histopathology. It updates previous findings with survey data on 509 primary antibodies and their staining conditions.

Keywords:
antibodyexperimental animalimmunohistochemistrytoxicological pathology

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Area of Science:

  • Veterinary Pathology
  • Histopathology
  • Immunohistochemistry

Background:

  • Immunohistochemistry (IHC) is crucial for histopathological examination in animal models.
  • Standardizing IHC protocols and antibody selection is essential for reproducible results.
  • Previous data on IHC antibodies was reported by the Conference on Experimental Animal Histopathology (CEAH) in 2017.

Purpose of the Study:

  • To share technical information on immunohistochemistry (IHC).
  • To facilitate the selection of suitable antibodies for histopathological examination in experimental animals.
  • To provide an updated resource on primary antibodies and their IHC staining conditions.

Main Methods:

  • A questionnaire was distributed to members of the Conference on Experimental Animal Histopathology (CEAH) between 2018 and 2019.
  • Data collected included immunological properties, supplier details, and IHC staining conditions for primary antibodies.
  • A total of 509 primary antibodies (220 different types) were analyzed.

Main Results:

  • Detailed immunological properties and supplier information were compiled for 509 primary antibodies.
  • Specific IHC staining conditions, including fixation, antigen retrieval, and antibody dilutions, were documented.
  • The results represent an update to the 2017 CEAH report, reflecting current practices.

Conclusions:

  • This technical report provides valuable, updated information on IHC antibodies and protocols for researchers.
  • The data facilitates informed antibody selection and optimization of IHC staining in animal histopathology.
  • Standardized technical data enhances the reliability and comparability of experimental animal histopathology studies.