ZFPM2-AS1 transcriptionally mediated by STAT1 regulates thyroid cancer cell growth, migration and invasion via

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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

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Ruizhen Ren1, Yuanna Du2, Xing Niu3

  • 1Department of Endocrinology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai 264000, Shandong, China.

Journal of Cancer
|May 12, 2021
PubMed

Insights

Long non-coding RNA ZFPM2-AS1 promotes thyroid cancer by acting as an oncogene. It is regulated by STAT1 and sponges miR-515-5p, inhibiting TUSC3 expression and enhancing cancer progression.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Thyroid cancer is a significant health concern.
  • Long non-coding RNAs (lncRNAs) play crucial roles in various cancers.
  • The specific role of ZFPM2 Antisense RNA 1 (ZFPM2-AS1) in thyroid cancer requires further elucidation.

Purpose of the Study:

  • To investigate the roles and molecular mechanisms of lncRNA ZFPM2-AS1 in thyroid cancer.
  • To analyze the functional impact of ZFPM2-AS1 on thyroid cancer cell behavior.
  • To elucidate the regulatory pathways involving ZFPM2-AS1, miR-515-5p, and TUSC3.

Main Methods:

  • Expression analysis of ZFPM2-AS1, miR-515-5p, and TUSC3 in thyroid cancer tissues and cells.
  • Functional assays including CCK-8, clone formation, EdU, ELISA, wound healing, and Transwell assays to assess cell proliferation, apoptosis, migration, and invasion.
  • Mechanism validation using Chromatin Immunoprecipitation (CHIP), pull-down, and luciferase reporter assays.

Main Results:

  • ZFPM2-AS1 and TUSC3 were upregulated, while miR-515-5p was downregulated in thyroid cancer.
  • Knockdown of ZFPM2-AS1 inhibited thyroid cancer cell growth, migration, and invasion.
  • ZFPM2-AS1, regulated by STAT1, acts as a sponge for miR-515-5p, reducing its inhibition on TUSC3 and promoting cancer progression.

Conclusions:

  • ZFPM2-AS1 functions as an oncogene in thyroid cancer.
  • STAT1 transcriptionally upregulates ZFPM2-AS1.
  • ZFPM2-AS1 promotes thyroid cancer malignancy by sponging miR-515-5p and affecting TUSC3 expression, suggesting its potential as a biomarker.
Keywords:
TUSC3ZFPM2-AS1miR-515-5pthyroid cancertranscription factor.

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