Transfection types, methods and strategies: a technical review

Area of Science:

• Molecular Biology
• Cell Biology
• Genetics

Background:

• Transfection is a key technique for introducing foreign nucleic acids into eukaryotic cells.
• This genetic modification is crucial for studying cellular processes, disease mechanisms, and gene therapy.
• Numerous transfection methods exist, each with unique characteristics.

Purpose of the Study:

• To review and compare different transfection methods.
• To analyze the strengths and limitations of various techniques.
• To provide guidance for selecting appropriate transfection strategies.

Main Methods:

• Literature review of reported transfection findings.
• Comparative analysis of transfection methods, nucleic acid types, controls, and efficiency assessment.
• Summarization of characteristics, strengths, and limitations.

Main Results:

• Detailed comparison of diverse transfection techniques.
• Evaluation of different nucleic acid types and their delivery.
• Assessment of transfection controls and efficiency measurement approaches.

Conclusions:

• Transfection is a versatile tool with broad applications in biological research and therapeutics.
• Understanding method-specific characteristics is vital for experimental success.
• This review aids researchers in choosing the most suitable transfection protocol.