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Updated: Nov 6, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
Optoproteomics elucidates the interactome of L-type amino acid transporter 3 (LAT3)
Liquan Wang1, Xiwen Sun1, Linjie Lu2
1The Second Afiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. wangliquan@zju.edu.cn.
Abstract:
Membrane protein interactions are crucial for diverse biological processes. We report the application of genetic code expansion in combination with photo-crosslinking chemistry, as we termed "optoproteomics", to identify proteins interacting with the human L-type membrane amino acid transporter 3 (LAT3, also known as SLC43A1). The site-specifically incorporated photo-cross-linker p-azido-L-phenylalanine (AzF), which reacts with proteins in their proximity, enabled the capture of weak and transient partners of LAT3 in living cells. We identify 11 unique interacting proteins which are light-sensitive and 19 unique proteins that are site-specific, validating the approach and providing insights into the LAT3 protein-protein interaction network currently unavailable.
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