[Methylene blue play a role in preventing septic liver injury by inducing macrophage polarization]
1Medical School of Nantong University, Nantong 226001, China Intensive Care Unit, Nantong Third People's Hospital, Nantong 226000, China.
Abstract:
Thirty mice were used to establish a sepsis model with cecal ligation and puncture. 15 mg/kg methylene blue or isotonic saline were injected intraperitoneally to observe liver tissue pathological changes. Changes in macrophage frequency and expressional condition of M1 and M2-type hepatic inflammatory factors were detected. After LPS stimulation, the expression level of macrophage inflammatory factor were detected. The results showed that the pathological liver injury was significantly reduced in the MB mice group (P < 0.05), and the frequency of liver macrophage was not statistically significantly different (P > 0.05). MB elevation had promoted the expression of M2-type hepatic inflammatory factor (P < 0.05) and macrophage inflammatory factor (P < 0.05). MB can play a role in preventing septic liver injury by inducing macrophages polarization to M2-type.
Insights
Methylene blue (MB) treatment significantly reduced liver injury in a mouse sepsis model. MB promoted M2 macrophage polarization, suggesting a protective role against septic liver damage.
Area of Science:
- Sepsis research
- Hepatology
- Immunology
Background:
- Sepsis can lead to multi-organ dysfunction, including liver injury.
- Hepatic macrophages play a critical role in regulating inflammatory responses during sepsis.
- Methylene blue (MB) has shown potential therapeutic effects in various conditions.
Purpose of the Study:
- To investigate the protective effect of methylene blue (MB) on septic liver injury.
- To explore the impact of MB on macrophage polarization and inflammatory factor expression in a mouse sepsis model.
Main Methods:
- A mouse model of sepsis was established using cecal ligation and puncture.
- Mice were treated with methylene blue (MB) or saline.
- Liver tissue pathology, macrophage frequency, and M1/M2 inflammatory factor expression were analyzed.
- Macrophage inflammatory factor expression was assessed after LPS stimulation.
Main Results:
- Methylene blue (MB) treatment significantly reduced pathological liver injury (P < 0.05).
- MB did not significantly alter the overall frequency of liver macrophages (P > 0.05).
- MB promoted the expression of M2-type hepatic inflammatory factors and macrophage inflammatory factors (P < 0.05).
Conclusions:
- Methylene blue (MB) demonstrates a protective effect against septic liver injury in mice.
- MB may exert its protective effects by inducing macrophage polarization towards the M2 phenotype.
