Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Regulation of spermatozoal capacitation by serotonin.

The Journal of reproduction and development·2026

Same author

Interactions among progesterone, estradiol, and γ-aminobutyric acid in rat spermatozoal hyperactivation.

The Journal of reproduction and development·2026

Same author

Associations Between Lifestyle Factors and Primary Dysmenorrhea in the Japan Nurses' Health Study.

Women's health reports (New Rochelle, N.Y.)·2025

Same author

Tryptophan promotes sperm hyperactivation in hamsters via 5-hydroxytryptamine biosynthesis within sperm.

The Journal of reproduction and development·2025

Same author

Association between incident occurrence and bed configuration: a retrospective observational study in a Japanese academic hospital during the COVID-19 pandemic.

BMJ leader·2025

Same author

Influences of 5-hydroxytriptamine on sperm hyperactivation and in vitro fertility in rats.

The Journal of reproduction and development·2025

Search research articles

Related Experiment Video

Updated: Nov 5, 2025

Measuring In Vivo Changes in Extracellular Neurotransmitters During Naturally Rewarding Behaviors in Female Syrian Hamsters

Measuring In Vivo Changes in Extracellular Neurotransmitters During Naturally Rewarding Behaviors in Female Syrian Hamsters

Published on: September 12, 2017

Serotonergic signals enhanced hamster sperm hyperactivation.

Chiyori Sakamoto¹, Masakatsu Fujinoki², Masafumi Kitazawa¹

¹Department of Obstetrics and Gynecology, School of Medicine, Dokkyo Medical University, Tochigi 321-0293, Japan.

The Journal of Reproduction and Development

|May 13, 2021

Summary

5-hydroxytryptamine (5-HT) enhances sperm hyperactivation via 5-HT2A and 5-HT4 receptors. These pathways involve specific signaling molecules like phospholipase C and adenylate cyclase, ultimately boosting sperm motility.

Keywords:

5-HT2 receptor 5-HT4 receptor 5-Hydroxytryptamine (5-HT)Hyperactivation Sperm

More Related Videos

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Published on: October 13, 2023

Related Experiment Videos

Last Updated: Nov 5, 2025

Measuring In Vivo Changes in Extracellular Neurotransmitters During Naturally Rewarding Behaviors in Female Syrian Hamsters

Measuring In Vivo Changes in Extracellular Neurotransmitters During Naturally Rewarding Behaviors in Female Syrian Hamsters

Published on: September 12, 2017

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Published on: October 13, 2023

Area of Science:

Reproductive biology
Molecular endocrinology
Sperm physiology

Background:

Sperm hyperactivation is crucial for fertilization.
5-hydroxytryptamine (5-HT) is known to influence sperm function.
The precise regulatory mechanisms of 5-HT-enhanced hyperactivation require elucidation.

Purpose of the Study:

To investigate the specific 5-hydroxytryptamine (5-HT) receptors involved in enhancing hamster sperm hyperactivation.
To identify the intracellular signaling pathways regulated by different 5-HT receptors during sperm hyperactivation.

Main Methods:

Utilized motility assays to assess sperm hyperactivation.
Administered agonists for various 5-HT receptors (5-HT2A, 5-HT4, 5-HT3).
Analyzed intracellular signaling molecules including phospholipase C (PLC), inositol 1,4,5-trisphosphate (IP3) receptors, soluble adenylate cyclase (sAC), transmembrane adenylate cyclase (tmAC), protein kinase A (PKA), and CatSper channels.

Main Results:

5-HT2A and 5-HT4 receptor agonists significantly enhanced sperm hyperactivation.
Specific 5-HT receptor agonists modulated sperm velocity and amplitude.
5-HT2A receptor stimulation involved PLC/IP3 signaling, while 5-HT4 receptor stimulation involved tmAC/PKA/CatSper channels.
Both pathways converge on sAC and PKA activation, leading to enhanced hyperactivation.

Conclusions:

5-hydroxytryptamine (5-HT) enhances sperm hyperactivation through distinct receptor-mediated pathways.
The 5-HT2A receptor activates PLC/IP3 signaling, and the 5-HT4 receptor activates tmAC/PKA/CatSper channels.
Both pathways ultimately converge on sAC and PKA, crucial for the regulation of hamster sperm hyperactivation.