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Updated: Nov 5, 2025
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
Published on: August 31, 2012
Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution
Bankala Krishnarjuna1, Toshio Yamazaki, G M Anantharamaiah
1Biophysics Program, Department of Chemistry, Biomedical Engineering, Macromolecular Science and Engineering, University of Michigan, Arbor, MI 48109, USA. ramamoor@umich.edu.
Abstract:
Cytochrome-P450-reductase transfers electrons to cytochrome-P450 through its flavin mononucleotide binding domain (FBD). Despite the importance of membrane-anchoring for FBD function, studies have focused on its soluble domain lacking the transmembrane-domain. Here we demonstrate that the reconstitution of FBD in nanodiscs enables high-resolution NMR measurements and renders a stable conformation.
