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Updated: Nov 5, 2025
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Published on: December 23, 2022
Sensitive, general and portable detection of RNAs combining duplex-specific nuclease transduction with an off-shelf
Lulu Guo1, Huan Li1, Rujian Zhao1
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China and Department of Chemistry, University of Science & Technology of China, Hefei, Anhui 230026, China.
Abstract:
We report a novel RNA sensing platform by combining the RNA:DNA hybridization, duplex-specific nuclease (DSN) amplification, and personal glucose meter (PGM) readout. The so-called DSN-PGM sensing platform is sensitive, specific and general to both microRNA and long virus RNA. The detection procedure is simple and instrument-free, thus holding particular potential in the development of portable and point-of-care measurements.
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