High Aspect Ratio and Light-Sensitive Micropillars Based on a Semiconducting Polymer Optically Regulate Neuronal...

Area of Science:

• Biomaterials Science
• Neuroscience
• Photonics

Background:

• Nano- and microstructured devices are explored for neuronal growth.
• Electrically conducting scaffolds with topographical cues are used.
• Optical stimulation combined with topographical cues for neuronal guidance is underexplored.

Purpose of the Study:

• Develop a light-addressable platform for modulating cellular growth.
• Investigate optical stimulation with topographical cues for neuronal guidance.
• Create a 3D platform using semiconducting polymers for embryonic cortical neurons.

Main Methods:

• Fabrication of high aspect ratio micropillars from regioregular poly(3-hexylthiophene-2,5-diyl) (P3HT).
• Culturing embryonic cortical neurons on P3HT micropillars.
• Applying visible light excitation to the P3HT platform.

Main Results:

• P3HT micropillars provide a mechanically compliant, neuron-compatible environment.
• Combined nano/microtopography and visible light effectively modulate neuronal growth and orientation.
• Optical excitation significantly increases neurite and axon length, inducing polarization.

Conclusions:

• A biocompatible, microstructured, light-sensitive platform enables wireless, controlled optical regulation of neuronal growth.
• This approach avoids genetic modification and has potential applications in regenerative medicine.
• The platform can be extended to other cell models, advancing photonic device applications.