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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Advances in Tumor Sampling and Sequencing in Breast Cancer and their Application in Precision Diagnostics and
Amos Chungwon Lee1,2, Han-Byoel Lee3,4,5, Huiran Yeom6
1Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, South Korea.
Abstract:
Intra- and Inter-tumoral heterogeneity is one of the main hurdles in diagnosing and treating breast cancer. Selecting, sampling, and sequencing the samples appropriately provide unique opportunities in realizing precision medicine. This chapter reviews some of the past landmarks, state-of-the-art technologies, and future directions of translational research in terms of tumor sampling technologies and sequencing in breast cancer. In the state-of-the-art technologies section, the technologies are categorized in terms of scientific, precision diagnostic, and precision therapeutic tools. Finally, limitations and future directions regarding various translational research for clinical applications using these technologies will be discussed.
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