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Published on: November 23, 2016
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Phospholipids (PLs) know-how: exploring and exploiting phospholipase D for its industrial dissemination
Peng Zhang1, Jin-Song Gong1, Jiufu Qin2
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, P. R. China.
Critical Reviews in Biotechnology
|May 14, 2021
Summary
Phospholipids (PLs) are vital nutraceuticals, but current extraction methods are inefficient. Microbial production using phospholipase D (PLD) offers a sustainable alternative, requiring improved enzyme properties and expression systems for industrial use.
Area of Science:
- Biotechnology
- Enzymology
- Biochemistry
Background:
- Phospholipids (PLs) are essential biological molecules with significant applications in nutraceuticals, food supplements, and cosmetics.
- Current PL extraction relies on soybean or egg yolk, facing challenges of high cost, inefficiency, and supply chain limitations.
- Microbial production via phospholipase D (PLD) offers a promising alternative to traditional PL extraction methods.
Purpose of the Study:
- To review recent advancements in phospholipase D (PLD) discovery, properties, and applications for phospholipid (PL) production.
- To highlight challenges in current PLD-based bioprocessing, including enzyme properties and microbial expression systems.
- To provide insights for future molecular engineering of PLD for enhanced PL bioproduction.
Main Methods:
- Systematic review of literature on phospholipase D (PLD) discovery and engineering.
- Analysis of structural properties and catalytic mechanisms of PLDs.
- Evaluation of expression strategies for enhancing PLD production in microbial systems.
- Summary of diverse applications of PLD-mediated bioprocessing for phospholipid production.
Main Results:
- Identification of diverse PLD enzyme variants with potential for improved properties.
- Assessment of various microbial expression systems for efficient PLD production.
- Overview of successful applications of PLD in phospholipid biotransformation.
- Highlighting limitations in current PLD technology hindering industrial scale-up.
Conclusions:
- Advancements in PLD discovery and engineering are crucial for overcoming limitations in phospholipid production.
- Improved enzyme properties and microbial expression systems are key to the industrial viability of PLD-mediated bioprocessing.
- Further research in molecular engineering of PLD will unlock its full potential for sustainable phospholipid manufacturing.
Keywords:
Biomanufacturingenzyme structuregenome and metagenome miningheterologous expressionmolecular engineeringphospholipase DphospholipidsMore Related Videos
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