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Updated: Nov 5, 2025

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
Proficiency at Tick Identification by Pathologists and Clinicians Is Poor.
Alvaro C Laga1, Scott R Granter1, Thomas N Mather2,3
1Department of Pathology, Brigham and Women's Hospital, and Harvard Medical School, Boston, MA.
Accurate tick identification is crucial for Lyme disease prevention. A survey revealed that most clinicians struggle to correctly identify various tick species, impacting appropriate prophylaxis administration.
Area of Science:
- Medical Entomology
- Infectious Diseases
- Public Health
Background:
- Accurate tick identification is essential for administering appropriate antimicrobial prophylaxis for Lyme disease.
- The northeastern United States has the highest incidence of Lyme disease.
- Clinician proficiency in tick identification directly impacts patient care and disease prevention strategies.
Purpose of the Study:
- To assess the proficiency of clinicians in identifying various tick species.
- To evaluate the accuracy of tick identification in a high-incidence Lyme disease region.
Main Methods:
- A voluntary survey was conducted among clinicians.
- High-resolution photographs of different tick species were used to test identification skills.
Main Results:
- Only 35% of ticks were correctly identified overall.
- While 60% could identify a nonengorged adult blacklegged tick, only 34% correctly identified a partially engorged one.
- Classification accuracy was significantly lower for brown dog, American dog, and Lone Star ticks.
Conclusions:
- Clinician proficiency in identifying tick species, including those that transmit Lyme disease, is alarmingly poor.
- This lack of proficiency may hinder the timely and correct administration of prophylaxis, potentially affecting patient outcomes.
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