Isotropically resolved label-free tomographic imaging based on tomographic moulds for optical trapping.

Area of Science:

• Microscopy and Imaging Science
• Optical Physics
• Biophysics

Background:

• Conventional 3D microscopy struggles with accurate spatial information and preserving native sample states.
• Axial resolution in traditional methods is limited by the inability to capture side scattering signals.

Purpose of the Study:

• To develop a 3D microscopy technique enabling isotropic resolution for free-floating samples.
• To overcome the limitations of optical tweezers in rotating complex-shaped specimens.

Main Methods:

• Utilized 3D structured light traps for stable optical rotation of complex-shaped, free-floating microscopic specimens.
• Measured side scattering information at various sample orientations to achieve isotropic resolution.

Main Results:

• Achieved an isotropic resolution of 230 nanometers.
• Successfully captured intricate structural details of colloidal multimers and live red blood cells.
• Demonstrated imaging capabilities beyond the scope of conventional tomographic microscopy.

Conclusions:

• The proposed optical rotation method significantly enhances 3D imaging capabilities.
• This technique offers a powerful tool for studying complex biological and material structures in their native states.
• Potential applications extend to various imaging challenges beyond the presented examples.