Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.3K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

499
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
499

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep learning enhanced quantitative phase imaging in digital holographic microscopy with attention empowered deep image prior.

Optics express·2026
Same author

ROS-mitochondria-NLRP3/STING axis mediates the enhanced anti-coronavirus activity of ZIF8 nanoparticles.

Journal of nanobiotechnology·2026
Same author

Interferometric image denoising network SEVReNet.

Biomedical optics express·2026
Same author

RS-N2N: a single-image phase denoising network for digital holographic microscopy.

Applied optics·2026
Same author

MCL1 promotes porcine epidemic diarrhea virus replication by modulating arachidonic acid metabolic pathway.

PLoS pathogens·2026
Same author

A novel TaqMan-based RT-qPCR assay for the detection of PEDV and discrimination of the G2c subtype.

Archives of virology·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Nov 5, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.1K

Limited-angle tomographic phase microscopy utilizing confocal scanning fluorescence microscopy.

Rongli Guo1, Itay Barnea1, Natan T Shaked1

  • 1Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978, Israel.

Biomedical Optics Express
|May 17, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a multimodal imaging method for accurate 3D refractive index (RI) profiling of live cells. By combining techniques, it overcomes data limitations for detailed cellular analysis.

More Related Videos

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.7K
Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

12.0K

Related Experiment Videos

Last Updated: Nov 5, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.1K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.7K
Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

12.0K

Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Imaging

Background:

  • Accurate 3D refractive index (RI) profiling is crucial for understanding live cell dynamics.
  • Traditional optical diffraction tomography struggles with limited projection data, leading to low-accuracy reconstructions.
  • Integrating morphological data can enhance RI tomogram reconstruction.

Purpose of the Study:

  • To develop a multimodal imaging technique for high-accuracy 3D RI profiling of live cells.
  • To overcome the challenge of missing projections in tomographic phase microscopy.
  • To combine molecular specificity with quantitative RI measurements within cells and organelles.

Main Methods:

  • Combines tomographic phase microscopy (TPM) with limited angular range and two-channel spinning-disk confocal scanning fluorescence microscopy.
  • Utilizes confocal fluorescence imaging to capture 3D cellular and organelle morphology.
  • Applies Gerchberg-Papoulis-based optical diffraction tomography (ODT) algorithm with morphological constraints.

Main Results:

  • Achieves high-accuracy 3D RI profiling of live cells even with sparse angular projection data.
  • Provides simultaneous measurement of cellular molecular specificity and internal RI distribution.
  • Demonstrates superior performance compared to conventional RI reconstruction methods.

Conclusions:

  • The proposed multimodal imaging technique enables accurate 3D RI mapping of live cells.
  • Integrating morphological constraints significantly improves RI reconstruction from limited projection data.
  • This method offers a powerful tool for detailed quantitative analysis of cellular structures and functions.