Related Experiment Video
Updated: Nov 5, 2025

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
Published on: December 4, 2020
Displacement and strain mapping for osteocytes under fluid shear stress using digital holographic microscopy and
Runyu Cao1, Wen Xiao1, Feng Pan1
1Key Laboratory of Precision Opto-mechatronics Technology, School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100083, China.
Abstract:
Osteocytes, as the mechano-sensors in bone, are always subjected to fluid shear stress (FSS) from the surrounding matrix. Quantification of FSS-induced cellular deformation is significant for clarifying the "perceive and transmit" process of cellular mechanotransduction. In this research, a label-free displacement and strain mapping method based on digital holographic microscopy (DHM) and digital image correlation (DIC) is introduced. The method, which is termed DHM-DIC, innovatively utilizes surface features extracted from holographic phase images instead of speckles as the metric for DIC searching. Simulation results on a hemisphere validate the feasibility of DHM-DIC. Displacement and strain maps of living osteocytes under 1.5 Pa FSS are evaluated from DHM-DIC and present good agreement with our previous finite element modeling results.
Related Concept Videos
Shearing Strain
Three-Dimensional Analysis of Strain
Measurements of Strain
Elastic Strain Energy for Shearing Stresses

