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YML018C protein localizes to the vacuolar membrane independently of Atg27p

Candyce M Sturgeon1, Nicholas Zanghi1, Hannah M Smith1

  • 1Department of Biology, High Point University, High Point, NC, USA 27268.

Micropublication Biology
|May 17, 2021
PubMed

Insights

The uncharacterized budding yeast YML018C protein localizes to the vacuolar membrane independently of the autophagy protein Atg27p. This finding clarifies the protein

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • The function of the budding yeast YML018C protein is currently unknown.
  • Previous studies suggest YML018C interacts with Atg27p and localizes to the vacuolar membrane, hinting at a role in autophagy.
  • The precise role of this interaction and YML018C in autophagy remains uncharacterized.

Purpose of the Study:

  • To investigate the functional relationship between YML018C and Atg27p.
  • To determine if Atg27p is necessary for the vacuolar membrane localization of YML018C.

Main Methods:

  • Utilized yeast genetics and fluorescence microscopy.
  • Observed YML018C localization in wild-type and atg27 deletion mutant strains of budding yeast.

Main Results:

  • The YML018C protein localizes to the vacuolar membrane.
  • Vacuolar membrane localization of YML018C was observed even in the absence of Atg27p.

Conclusions:

  • Atg27p is not required for the vacuolar membrane localization of the YML018C protein.
  • This finding suggests that the interaction between YML018C and Atg27p, if it occurs, is not essential for targeting YML018C to the vacuolar membrane.

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