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Updated: Nov 5, 2025

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
Photoactivatable fluorophores for durable labelling of individual cells
Hiroki Kashima1, Mako Kamiya1, Fumiaki Obata2
1Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. mkamiya@m.u-tokyo.ac.jp uranokun@m.u-tokyo.ac.jp.
Abstract:
We have designed and developed non-fluorescent, cell-permeable photoactivatable fluorophores, photoactivatable SPiDERs (paSPiDERs), which exhibit fluorescence activation upon light irradiation, accompanied by the generation of a quinone methide intermediate that binds covalently to intracellular proteins. The fluorescence signal is durable for 24 hours, resistant to fixation and compatible with immunostaining, and selective cell labeling can be achieved at single-cell resolution.
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