Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Temporal information encoding in isolated cortical networks.

Cerebral cortex (New York, N.Y. : 1991)·2025

Same author

Cell-generated mechanical forces play a role in epileptogenesis after injury.

bioRxiv : the preprint server for biology·2025

Same author

Advances in Recapitulating Alzheimer's Disease Phenotypes Using Human Induced Pluripotent Stem Cell-Based In Vitro Models.

Brain sciences·2022

Same author

Mitochondrial complex I as a therapeutic target for Alzheimer's disease.

Acta pharmaceutica Sinica. B·2022

Same author

Microdevice for directional axodendritic connectivity between micro 3D neuronal cultures.

Microsystems & nanoengineering·2021

Same author

Micro Three-Dimensional Neuronal Cultures Generate Developing Cortex-Like Activity Patterns.

Frontiers in neuroscience·2020

Same journal

Salt priming coordinates transcriptional and epigenetic states for enhanced salt tolerance in mung bean (Vigna radiata).

Communications biology·2026

Same journal

A male-derived volatile sex pheromone in Caenorhabditis nematodes identified through its mimicry by a predator.

Communications biology·2026

Same journal

Revalidation of Manis aurita based on integrative genomic and morphological evidence.

Communications biology·2026

Same journal

Presenilin-1 controls glycolysis and identity of pancreatic beta cells.

Communications biology·2026

Same journal

Base editing-derived models of human WDR34 and WDR60 disease alleles replicate retrograde intraflagellar transport (IFT) and hedgehog signaling defects.

Communications biology·2026

Same journal

Butterflies with low thermoregulatory capacity show greatest upwards range shifts along an elevational gradient.

Communications biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 5, 2025

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

Neuron and astrocyte aggregation and sorting in three-dimensional neuronal constructs.

Md Fayad Hasan¹, Yevgeny Berdichevsky^2,3

¹Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA, USA.

Communications Biology

|May 18, 2021

Summary

This summary is machine-generated.

This study reveals how neurons and astrocytes aggregate and sort in 3D cultures, crucial for engineered neural tissues. Findings show neuron-driven aggregation and astrocyte sheath formation, impacting neural circuit function.

More Related Videos

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Published on: August 16, 2018

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Published on: November 14, 2016

Related Experiment Videos

Last Updated: Nov 5, 2025

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Published on: August 16, 2018

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Published on: November 14, 2016

Area of Science:

Neuroscience
Biomedical Engineering
Computational Biology

Background:

Cell aggregation and sorting are known in non-neuronal 3D cultures.
Limited data exists on collective cell movements in engineered neural tissues.
Cellular rearrangement can impact neural construct morphology and function.

Purpose of the Study:

To investigate neuronal and astrocyte aggregation and sorting in 3D cultures.
To develop a computational model for these cellular dynamics.
To understand how these processes influence engineered neural tissue design.

Main Methods:

Development of linear, adhered 3D neuronal-astrocyte cultures (rat and human).
Creation of an in silico model simulating contraction, clustering, and cell sorting.
Experimental observation and computational analysis of cell behaviors.

Main Results:

Aggregation in 3D cultures is primarily neuron-mediated.
Differential attraction between neurons and astrocytes drives astrocyte sheath formation.
In silico models predicted self-assembled neuronal layers in disk-shaped cultures.
Neuronal activity correlated with local morphological variations.

Conclusions:

Neuron-astrocyte interactions govern aggregation and sorting in 3D neural constructs.
The developed model aids in understanding and designing functional engineered neural tissues.
This research provides insights into the self-organization of neural cells in vitro.