Related Experiment Video
Updated: Nov 5, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
Published on: April 19, 2021
iPick: Multiprocessing software for integrated NMR signal detection and validation
Mehdi Rahimi1, Yeongjoon Lee1, John L Markley2
1Department of Chemistry, University of Colorado Denver, Denver, CO 80204, USA.
Abstract:
Peak picking is a critical step in biomolecular NMR spectroscopy. The program, iPick, presented here provides a scripting tool and a graphical user interface (GUI), which allow the user to perform interactive and intuitive peak picking and validation. The click-and-run GUI requires no computer programming skills, while the scripting tool can be used by more advanced users to customize the application. If used with a multi-core CPU, the multiprocessing feature of iPick reduces the processing time significantly by invoking parallel computing. The GUI is a plugin, compatible with the popular NMRFAM-SPARKY software package and its newly released successor, the POKY software. Features implemented in iPick include automated noise level detection and threshold setting, cross-validation against multiple spectra, and a method for quantifying peak reliability. The iPick software is cross-platform, open-source, and freely available from https://github.com/pokynmr/ipick.
More Related Videos
07:24Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
Published on: September 23, 2021
09:25Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
Published on: November 1, 2024
Related Concept Videos
Applications Of NMR In Biology
NMR Spectrometers: Overview
¹H NMR Signal Integration: Overview
¹H NMR Signal Multiplicity: Splitting Patterns
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...