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Updated: Nov 5, 2025

BEST: Barcode Enabled Sequencing of Tetrads
Published on: May 1, 2014
ChIP-Sequencing of TET Proteins
Kasper D Rasmussen1, Kristian Helin2,3,4
1Centre for Gene Regulation & Expression, School of Life Sciences, University of Dundee, Dundee, UK.
This study details a ChIP-seq protocol to map DNA interactions with TET2, a key enzyme in DNA methylation. This method helps understand TET protein function in specific cellular contexts.
Area of Science:
- Biochemistry
- Molecular Biology
- Epigenetics
Background:
- TET proteins are methylcytosine dioxygenases crucial for regulating the DNA methylation landscape.
- Understanding TET protein interactions with chromatin is vital for deciphering their cellular functions.
Purpose of the Study:
- To present a detailed ChIP-seq protocol for analyzing TET2-bound DNA.
- To provide a method adaptable for studying other TET enzymes.
Main Methods:
- Chromatin immunoprecipitation sequencing (ChIP-seq) protocol.
- Utilizes disuccinimidyl glutarate (DSG) and formaldehyde crosslinking.
- Focuses on analyzing TET2-bound DNA.
Main Results:
- The protocol effectively maps DNA regions bound by TET2.
- Demonstrates the utility of DSG and formaldehyde crosslinking for TET protein studies.
Conclusions:
- This ChIP-seq protocol offers a robust method for investigating TET protein-DNA interactions.
- Facilitates deeper understanding of TET enzyme roles in epigenetic regulation.
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