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Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing
P J Jackson1, C J Sampson, E H Cooper
1Unit for Cancer Research, University of Leeds, UK.
Annals of Clinical Biochemistry
|May 1, 1988
Abstract:
We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.