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Related Experiment Video

Updated: Nov 5, 2025

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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Correlation between circulating blood and microenvironment T lymphocytes in diffuse large B-cell lymphomas.

Filomena Emanuela Laddaga1, Giuseppe Ingravallo2, Anna Mestice3

  • 1Clinical Pathology Unit, AOU Consorziale Policlinico, Bari, Italy.

Journal of Clinical Pathology
|May 20, 2021
PubMed
Summary

Patients with diffuse large B-cell lymphoma (DLBCL) and higher T-cell infiltration in their tumors show a better response to therapy. Lower T-cell counts in blood and tumors correlate with chemoresistance in DLBCL patients.

Keywords:
lymphocyte countlymphocyteslymphoma

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Area of Science:

  • Immunology
  • Oncology
  • Hematology

Background:

  • Diffuse large B-cell lymphoma (DLBCL) exhibits significant clinical and biological heterogeneity.
  • This heterogeneity is influenced by both tumor cells and the surrounding tumor microenvironment (TME).

Purpose of the Study:

  • To investigate circulating lymphocyte and monocyte subsets.
  • To explore their relationship with T cells within the TME in DLBCL patients.
  • To differentiate between chemoresistant and chemosensitive DLBCL cases.

Main Methods:

  • Analysis of circulating lymphocyte and monocyte subsets in DLBCL patients.
  • Assessment of T cell infiltration within the tumor microenvironment.
  • Correlation of peripheral blood cell counts and ratios with TME T cells.

Main Results:

  • Chemoresistant DLBCL patients had lower absolute lymphocyte counts (ALC) and CD3+, CD4+ T cells compared to chemosensitive patients.
  • Lymphocyte:monocyte ratio (LMR) positively correlated with peripheral blood CD3+ and CD4+ T cells.
  • ALC, LMR, and peripheral blood CD3+, CD4+ T cells positively correlated with T cell infiltration in the TME.

Conclusions:

  • High TME T cell infiltration in DLBCL suggests a pre-existing anti-tumor immune response.
  • Rituximab-based regimens further stimulate TME T cells in DLBCL.
  • Low T-cell infiltration in DLBCL indicates a lack of anti-tumor immunity and poorer treatment response.