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Related Experiment Video

Updated: Nov 5, 2025

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
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Antifungal Susceptibility Testing and Identification.

Sarah E Kidd1, Lucy C Crawford2, Catriona L Halliday3

  • 1National Mycology Reference Centre, Microbiology & Infectious Diseases, SA Pathology, SA Pathology (Frome Campus), PO Box 14, Rundle Mall, Adelaide, South Australia 5000, Australia; School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia.

Infectious Disease Clinics of North America
|May 21, 2021
PubMed
Summary

Antifungal susceptibility testing is crucial for treating fungal infections, especially with new drugs and resistant strains. Standardized methods and user training are vital for accurate results to guide patient therapy effectively.

Keywords:
Antifungal susceptibility testingCLSIClinical breakpoints (CBP)EUCASTEpidemiologic cutoff values (ECV)Fungal barcodingMALDI-ToFSensititre YeastOne

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Area of Science:

  • Medical Mycology
  • Clinical Microbiology
  • Antimicrobial Resistance

Background:

  • Increasing incidence of fungal infections, particularly in at-risk populations.
  • Emergence of antifungal-resistant fungal strains necessitates updated treatment strategies.
  • Growing availability of novel antifungal agents requires robust susceptibility testing.

Purpose of the Study:

  • To highlight the growing importance of antifungal susceptibility testing (AFST).
  • To discuss challenges and essential requirements for accurate AFST.
  • To emphasize the need for standardization and user training in AFST.

Main Methods:

  • Review of current antifungal susceptibility testing methodologies.
  • Discussion on factors influencing variability in AFST results.
  • Emphasis on the importance of standardization and validation protocols.

Main Results:

  • AFST methods have improved but show inter-method variability.
  • Standardization, validation, and user training are critical for clinical utility.
  • Lack of comprehensive interpretive criteria for many drug-species combinations.

Conclusions:

  • Accurate fungal identification and susceptibility testing are paramount for guiding antifungal therapy.
  • Standardized and validated AFST methods with trained personnel are essential.
  • Ongoing development of interpretive criteria is necessary for effective clinical application of AFST.