Complete Genome Sequence of Burkholderia gladioli Myophage Mana
Brenda Godoy1, Guichun Yao1, Tram Le1
1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
Microbiology Resource Announcements
|May 21, 2021
Abstract:
Burkholderia gladioli is known to cause respiratory tract infections in cystic fibrosis patients. Here, we describe the annotation of the 38,038-bp genome sequence of Mana, a P2-like phage of B. gladioli Understanding the genomic characteristics of phages infecting pathogens like B. gladioli can lead to advancements in phage therapy.
Insights
Researchers sequenced the genome of the Mana phage, which infects Burkholderia gladioli. This genomic information advances understanding of phages and supports potential phage therapy applications.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Burkholderia gladioli is a pathogen causing respiratory infections, particularly in cystic fibrosis patients.
- Bacteriophages are viruses that infect bacteria and are being explored for therapeutic uses.
Purpose of the Study:
- To annotate the genome sequence of the Mana phage, a P2-like phage infecting B. gladioli.
- To provide genomic insights into phages targeting B. gladioli for potential therapeutic applications.
Main Methods:
- Whole-genome sequencing of the Mana phage.
- Bioinformatic analysis for genome annotation.
Main Results:
- The genome sequence of the Mana phage was fully annotated.
- The phage genome size was determined to be 38,038 base pairs.
Conclusions:
- The genomic characterization of the Mana phage provides a foundation for understanding its biology.
- This research contributes to the development of phage therapy strategies against B. gladioli infections.
Related Concept Videos
01:26DNA Bacteriophages
343
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
343
01:30Lytic Cycle of Bacteriophages
73.8K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
73.8K
00:43Lysogenic Cycle of Bacteriophages
64.7K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
64.7K
01:24Bacterial Phylum Tenericutes
198
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
198
01:20Viral Replication: Lytic Cycle
612
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
612
01:16Viral Replication: Lysogenic Cycle
599
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
599