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MYC Analysis in Cancer and Evolution.

Markus Hartl1, Klaus Bister2

  • 1Institute of Biochemistry, Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria. markus.hartl@uibk.ac.at.

Methods in Molecular Biology (Clifton, N.J.)
|May 21, 2021
PubMed
Summary
This summary is machine-generated.

The MYC oncogene drives cancer by deregulating cell growth and division. This chapter details methods to study MYC

Keywords:
CancerCell transformationEvolutionOncogeneProtein–protein and protein–DNA interactionsTranscription factorTumor suppressor

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Area of Science:

  • Oncogenomics
  • Molecular Biology
  • Cancer Research

Background:

  • The MYC oncogene (v-myc) was first identified in avian retroviruses.
  • Cellular MYC (c-myc) protein is central to gene expression networks regulating cell growth, metabolism, proliferation, differentiation, and apoptosis.
  • MYC deregulation is a common cause of human cancers.

Purpose of the Study:

  • To present experimental systems and strategies for evaluating MYC's oncogenic potential.
  • To describe methods for testing MYC inhibitors.
  • To outline approaches for studying MYC protein interactions and evolutionary origins.

Main Methods:

  • Cellular systems for quantifying oncogenic potential.
  • Assays for testing MYC inhibitors.
  • Techniques for monitoring MYC protein-protein interactions.
  • Procedures for studying MYC evolutionary origins, structure, function, and regulation.

Main Results:

  • The chapter provides a framework for comprehensive MYC research.
  • Established methods allow for quantitative assessment of MYC's role in cell transformation.
  • Experimental strategies cover inhibitor testing, interaction monitoring, and evolutionary analysis.

Conclusions:

  • Understanding MYC's multifaceted roles is crucial for cancer research.
  • The described methodologies facilitate deeper insights into MYC's oncogenic mechanisms.
  • This work supports the development of targeted cancer therapies.