Measuring MYC-Mediated Metabolism in Tumorigenesis
Hsin-Yao Tang1, Aaron R Goldman2, Xue Zhang2,3
1Systems and Computational Biology Center and Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA, USA. tangh@Wistar.org.
Methods in Molecular Biology (Clifton, N.J.)
|May 21, 2021
Summary
The MYC gene is crucial for normal cell growth but its deregulation drives cancer progression. This chapter highlights MYC
Area of Science:
- Molecular Biology
- Cancer Biology
- Genetics
Background:
- The MYC gene plays a vital role in regulating normal cellular growth and proliferation.
- Deregulation of MYC is implicated in the development and progression of numerous human cancers.
Purpose of the Study:
- To elucidate the role of the MYC transcription factor in cellular growth and cancer.
- To highlight the contribution of metabolomics in understanding MYC's function.
Main Methods:
- RNA sequencing
- Genome-wide chromatin immunoprecipitation
- Proteomics
- Metabolomics
Main Results:
- MYC transcription factor activates housekeeping genes involved in essential cellular processes.
- These processes include ribosome biogenesis, synthesis of fatty acids, proteins, and nucleotides.
- MYC influences nutrient uptake, waste removal, glycolysis, and glutamine metabolism.
Conclusions:
- MYC is a key driver of cell growth, with its deregulation contributing to tumorigenesis.
- Metabolomics provides critical insights into MYC's multifaceted role in cellular metabolism and cancer.
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