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Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...

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Updated: Nov 4, 2025

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Methods to Study Myc-Regulated Cellular Senescence: An Update.

Fan Zhang^1,2, Wesam Bazzar¹, Mohammad Alzrigat¹

¹Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Methods in Molecular Biology (Clifton, N.J.)

|May 21, 2021

Summary

This summary is machine-generated.

Cellular senescence, a barrier against cancer, is regulated by MYC. New methods allow simultaneous detection of multiple senescence markers, aiding research into MYC

Keywords:

Cellular senescence EdU MUG assay MYC Phalloidin SA-β-gal Sudan Black B GL13

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Last Updated: Nov 4, 2025

Techniques to Induce and Quantify Cellular Senescence

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Published on: August 12, 2018

Area of Science:

Cellular biology
Cancer research
Aging research

Background:

Cellular senescence is crucial in aging, development, and tumor suppression.
The MYC protein is implicated in regulating senescence and tumor development.
Accurate senescence detection requires multiple markers due to the lack of a single definitive marker.

Purpose of the Study:

To describe protocols for simultaneous in situ detection of multiple senescence markers.
To present a quantitative fluorogenic method for senescence-associated β-galactosidase (SA-β-gal) activity.
To introduce a novel method using a Sudan Black B (SBB) analogue (GL13) for senescent cell detection in fixed tissues.

Main Methods:

Development of protocols for simultaneous in situ detection of multiple senescence markers.
Quantitative fluorogenic assay for senescence-associated β-galactosidase (SA-β-gal) activity.
GL13 staining method for senescent cells in formalin-fixed paraffin-embedded tissues.

Main Results:

Established protocols for simultaneous, multi-marker senescence detection.
Validated a quantitative SA-β-gal activity assay.
Demonstrated the utility of GL13 for senescent cell identification in fixed tissues.

Conclusions:

The developed methods enable comprehensive analysis of cellular senescence.
These tools will advance understanding of MYC's role in senescence regulation.
Further research using these methods will clarify senescence's impact on physiology and cancer.