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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
The isolation, purification and complete characterization of the diterpene forskolin from nutritional supplements
Alexander O Pritchard1, Dion B Nemez1, David E Herbert1
1Department of Chemistry, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
Abstract:
Forskolin (1) is a diterpene found in the Coleus forskohlii plant that has been examined for its medical properties resulting from adenylyl cyclase activation. This article describes a straightforward purification method of 1 from commercially available weight loss capsules. In addition, there has been some ambiguity with respect to the use of the name 'forskolin' to describe 1 and related diterpenes, which this report serves to eliminate. Herein we detail the complete spectroscopic characterization of purified 1 as well as its single crystal X-ray structure.
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