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Mineral analysis in experimental corneal scars. An EDAX study
S Bonafonte1, J N Fernandez del Cotero, A Aguirre Vila-Coro
1Centro de Oftalmologia Pelayo, Barcelona, Spain.
Cornea
|January 1, 1988
Summary
This study found that calcium is the only element that increases in corneal scar tissue compared to the surrounding cornea after injury. This research utilized scanning electron microscopy and energy dispersive analysis of x-ray (EDAX) for elemental analysis.
Area of Science:
- Ophthalmology
- Materials Science
- Biochemistry
Background:
- Corneal wound healing involves complex biological and chemical processes.
- Understanding the elemental composition of scar tissue is crucial for developing targeted treatments.
Purpose of the Study:
- To investigate the inorganic elemental composition of corneal scar tissue.
- To compare the elemental profile of scar tissue with the surrounding healthy cornea.
Main Methods:
- Inducing central penetrating excisional wounds in rabbit and trout corneas.
- Employing scanning electron microscopy and energy dispersive analysis of x-ray (EDAX) for elemental analysis.
- Performing semiquantitative determination of inorganic elements (atomic numbers 9-93).
Main Results:
- Calcium was identified as the only element with a higher concentration in the healing corneal wound compared to the surrounding cornea.
- Relative concentrations of other inorganic elements were compared between scar and healthy corneal tissue.
Conclusions:
- Corneal wound healing is associated with a localized increase in calcium.
- The EDAX technique provides a valuable method for analyzing inorganic elements in corneal tissue during healing.