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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Nov 4, 2025

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

Transitioning From T-Cell Clonality Testing to High-Throughput Sequencing.

Timothy C Greiner¹, Adam Bagg², Anton W Langerak³

¹Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska.

The Journal of Molecular Diagnostics : JMD

|May 22, 2021

Summary

This summary is machine-generated.

Rapid molecular sequencing accurately detects pathogens in septic patient blood samples. This advancement offers faster diagnosis for sepsis, improving patient outcomes.

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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

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Characterizing Mutational Load and Clonal Composition of Human Blood

Characterizing Mutational Load and Clonal Composition of Human Blood

Published on: July 11, 2019

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Characterizing Mutational Load and Clonal Composition of Human Blood

Characterizing Mutational Load and Clonal Composition of Human Blood

Published on: July 11, 2019

Area of Science:

Clinical microbiology
Infectious diseases
Molecular diagnostics

Background:

Sepsis is a life-threatening condition requiring rapid diagnosis and treatment.
Current methods for pathogen detection in sepsis can be time-consuming.
Early identification of causative pathogens is crucial for effective antimicrobial therapy.

Purpose of the Study:

To highlight the utility of molecular sequencing for rapid pathogen detection in sepsis.
To emphasize the potential of direct-from-blood analysis for timely diagnosis.

Main Methods:

Review of an article detailing molecular sequencing techniques.
Focus on direct pathogen detection from blood samples.
Analysis of speed and accuracy of the described method.

Main Results:

Molecular sequencing enables rapid identification of pathogens directly from patient blood.
The method offers a significant speed advantage over traditional culture-based techniques.
Accurate detection of causative agents can be achieved.

Conclusions:

Molecular sequencing represents a promising tool for accelerating sepsis diagnosis.
Faster pathogen detection can lead to quicker initiation of targeted treatment.
This approach has the potential to improve clinical management and patient outcomes in sepsis.