Molecular cloning and expression analysis of a WRKY transcription factor gene, GbWRKY20, from Ginkgo biloba

WRKY transcription factors are important regulators of diverse plant life processes. Our aim was to clone and characterize GbWRKY20, a WRKY gene of group IIc, derived from Ginkgo biloba. The cDNA sequence of GbWRKY20 was 818 bp long, encoding a 271-amino acid proteins and containing two introns and three exons. The proteinic molecular weight was 30.99 kDa, with a relevant theoretical isoelectric point of 8.15. Subcellular localization analysis confirmed that the GbWRKY20 protein localized to the nucleus. In total, 75 cis-regulatory elements of 19 different types were identified in the GbWRKY20 promoter sequence, including some elements involved in light responsiveness, anaerobic induction and circadian control, low-temperature responsiveness, as well as salicylic acid (SA) and auxin responsiveness. Expression pattern analysis of plant samples from different developmental stages and tissue types, revealed differential GbWRKY20 expression. The GbWRKY20 transcript was downregulated 12 h after heat treatment and at 4-12 h after drought treatment, but was upregulated 12 h after NaCl, cold and methyl jasmonate treatments. For abscisic acid and SA treatments, the GbWRKY20 transcript was upregulated at 24 h. In summary, GbWRKY20 encoded a newly cloned WRKY transcription factor of G. biloba that might be involved in plant growth and plant responses to abiotic stresses and hormones treatments.