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Updated: Nov 4, 2025

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos
Published on: October 3, 2018
Generation of gene-level resolution chromosome contact maps in bacteria and archaea
Charlotte Cockram1, Agnès Thierry1, Romain Koszul1
1Institut Pasteur, Unité Régulation Spatiale des Génomes, CNRS UMR 3525, 5015 Paris, France.
Abstract:
Chromosome conformation capture (Hi-C) has become a routine method for probing the 3D organization of genomes. However, when applied to bacteria and archaea, current protocols are expensive and limited in their resolution. By dissecting the different steps of published eukaryotic and prokaryotic Hi-C protocols, we have developed a cost- and time-effective approach to generate high-resolution (down to 500 bp - 1 kb) contact matrices of both bacteria and archaea genomes. For complete details on the use and execution of this protocol, please refer to Cockram et al. (2020).
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