Spatial multi-omics sequencing for fixed tissue via DBiT-seq

Area of Science:

• Spatial omics
• Molecular biology
• Genomics

Background:

• Spatial omics technologies are crucial for understanding tissue architecture and cellular function.
• Existing methods may have limitations in resolution or multiplexing capabilities for complex tissues.

Purpose of the Study:

• To describe a protocol for deterministic barcoding in tissue (DBiT) for spatial omics.
• To enable the construction of multi-omics atlases from fixed frozen tissue samples.
• To achieve near-single-cell resolution for spatial transcriptomics and targeted protein analysis.

Main Methods:

• Utilizes a microfluidic-based approach to deliver combinatorial DNA oligo barcodes to cells within a tissue section.
• Applies the technique to fixed frozen tissue samples mounted on a glass slide.
• The method achieves near-single-cell resolution without direct single-cell dissociation.

Main Results:

• The DBiT platform allows for spatial transcriptomics analysis.
• Enables spatial analysis of targeted protein panels within the tissue context.
• Facilitates the creation of comprehensive multi-omics atlases.

Conclusions:

• The described protocol provides a robust method for spatial multi-omics analysis on fixed frozen tissues.
• The DBiT approach offers near-single-cell resolution, advancing spatial biology research.
• This technique is valuable for constructing detailed tissue atlases and understanding spatial molecular organization.