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ZEISS Airyscan: Optimizing Usage for Fast, Gentle, Super-Resolution Imaging.

Xufeng Wu1, John A Hammer2

  • 1Light Microscopy Facility, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA. wux@nhlbi.nih.gov.

Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2021
PubMed
Summary

The Zeiss Airyscan microscope enhances confocal microscopy to super-resolution imaging. It significantly improves image resolution and signal quality for cell biology research.

Keywords:
3D imagingAiryscanConfocal microscopySuper-resolutionZeiss 880

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Area of Science:

  • Microscopy
  • Cell Biology
  • Super-resolution Imaging

Background:

  • Conventional confocal microscopes have limitations in resolution.
  • The Zeiss Airyscan utilizes a specialized detector to overcome these limits.
  • Airyscan is an add-on to Zeiss laser scanning confocal microscopes.

Purpose of the Study:

  • To explain the fundamentals of Zeiss Airyscan imaging.
  • To guide users in optimizing acquisition settings and image processing.
  • To provide best practices for Airyscan microscopy on Zeiss 800 series.

Main Methods:

  • Utilizing a 32-channel Airyscan detector.
  • Transforming a confocal microscope into a super-resolution system.
  • Focusing on Airyscan function over conventional confocal mode.

Main Results:

  • Achieved twofold improvement in resolution.
  • Obtained eightfold increase in signal-to-noise ratio.
  • Maintained confocal functionality alongside super-resolution capabilities.

Conclusions:

  • Zeiss Airyscan is a powerful tool for super-resolution microscopy.
  • Proper settings and processing are crucial for optimal image quality.
  • This guide facilitates effective use of Airyscan for cell biologists.