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Anti-angiogenesis Function of Ononin via Suppressing the MEK/Erk Signaling Pathway

Guowei Gong1, Yuzhong Zheng2, Xiangpeng Kong3

  • 1Department of Bioengineering, Zunyi Medical University, Zhuhai Campus, Zhuhai, Guangdong 519041, China.

Journal of Natural Products
|May 24, 2021
PubMed

Insights

Ononin, a plant-derived flavonoid, effectively inhibits angiogenesis, the process crucial for tumor development. This study shows ononin suppresses endothelial cell migration, invasion, and tube formation, offering potential for cancer prevention.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Angiogenesis is a critical pathological process in tumor development.
  • Flavonoids, like ononin, are explored for chemopreventive properties.
  • Ononin's anti-angiogenic effects remain largely unexplored.

Purpose of the Study:

  • To investigate the anti-angiogenic properties of ononin in cultured endothelial cells.
  • To elucidate the molecular mechanisms underlying ononin's potential anti-angiogenic effects.

Main Methods:

  • Human Umbilical Vein Endothelial Cells (HUVECs) were treated with ononin.
  • Assays measured cell migration, invasion, and tube formation.
  • Apoptosis markers and key protein expression levels (HIF-1α, MEK/ERK, VEGFR2) were analyzed.

Main Results:

  • Ononin significantly suppressed VEGF-induced HUVEC migration, invasion, and tube formation.
  • Ononin treatment enhanced apoptosis by increasing the Bax/Bc-2 ratio and cleaved caspase levels.
  • Ononin reduced the expression of HIF-1α, MEK/ERK, and VEGFR2 proteins.

Conclusions:

  • Ononin exhibits significant anti-angiogenic activity by inhibiting endothelial cell functions.
  • Ononin promotes apoptosis and modulates key signaling pathways involved in angiogenesis.
  • Ononin represents a potential therapeutic candidate for preventing and treating angiogenesis-related diseases, including cancer.

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