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Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

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FRET-Based Probe for High-Throughput DNA Intercalator Drug Discovery and In Vivo Imaging.

Chandrashekhar U Murade¹, Samata Chaudhuri¹, Ibtissem Nabti¹

¹Physics Program, New York University Abu Dhabi, Abu Dhabi 129188, United Arab Emirates.

|May 24, 2021

Summary

Researchers developed a novel FRET-based probe for rapid screening of DNA intercalating drugs. This tool identifies known and new intercalators, aiding cancer therapy and antimicrobial discovery.

Keywords:

DNA intercalators antimicrobials cancer therapy drug discovery drug screen oligo probe sensor

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Area of Science:

Biochemistry and Molecular Biology
Drug Discovery and Development
Genetics and Genomics

Background:

DNA intercalators are crucial in cancer therapy and antimicrobial treatments due to their interference with DNA processing.
Accelerating the discovery of novel DNA intercalating agents is essential for advancing these fields.

Purpose of the Study:

To design and validate a fluorescence resonance energy transfer (FRET)-based probe for high-throughput screening of DNA intercalating compounds.
To enable rapid identification and characterization of novel and known DNA intercalators both in vitro and in vivo.

Main Methods:

Development of a FRET-based probe for detecting DNA intercalation.
High-throughput screening of chemical libraries using the FRET probe.
Cellular studies to image drug entry and interaction with DNA in situ.
Correlation of drug potency with cellular penetration and DNA interaction.

Main Results:

The FRET probe successfully identified known DNA intercalators from approved drug libraries.
Previously unreported intercalating compounds were discovered using the probe.
The probe facilitated direct imaging of drug-DNA interactions within the cell nucleus.
A correlation was established between intercalator potency against cancer cells and their ability to penetrate cell membranes.

Conclusions:

The developed FRET probe is a sensitive and rapid tool for identifying DNA intercalators.
The probe's ability to function in vitro and in vivo accelerates the discovery and repurposing of DNA-intercalating drugs.
This technology holds significant potential for advancing cancer therapies and antimicrobial treatments.