Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

12.3K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.3K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.0K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.0K
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.8K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.8K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

23.5K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
23.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural and biochemical basis for cannabinoid cyclase activity in marine bacterial flavoenzymes.

Nature chemical biology·2026
Same author

Bioluminescent Probes for Multiplexed RNA Imaging.

Journal of the American Chemical Society·2026
Same author

Fluorescent Protein Chromophore-Based Luciferins for Bioluminescence Imaging.

Chemical & biomedical imaging·2025
Same author

Semisynthetic, Multicolor Probes for Bioluminescence Imaging.

ACS omega·2025
Same author

Continuous Hypermutation and Evolution of Luciferase Variants.

ACS chemical biology·2025
Same author

Structural and biochemical basis for cannabinoid cyclase activity in marine bacterial flavoenzymes.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Nov 4, 2025

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.2K

Caged Cumate Enables Proximity-Dependent Control Over Gene Expression.

Anna C Love1, Sabrina H Tran2, Jennifer A Prescher1,3,4

  • 1Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA 92697, USA.

Chembiochem : a European Journal of Chemical Biology
|May 25, 2021
PubMed
Summary

Researchers developed a novel method to visualize cell proximity using transcriptional activators. This system detects when cells are close, enabling new ways to study cellular interactions.

Keywords:
caged probecellular contactcumategene transcriptionimaging

More Related Videos

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.6K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.6K

Related Experiment Videos

Last Updated: Nov 4, 2025

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.2K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.6K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.6K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Cell-cell interactions are crucial for physiological functions.
  • Conventional imaging methods face limitations in studying cellular proximity.
  • Understanding direct cell-to-cell communication is essential in biological research.

Purpose of the Study:

  • To develop a novel strategy for imaging and potentially manipulating cell proximity.
  • To create a system that visually indicates when cells are in close contact.
  • To overcome limitations of current methods for observing cellular interactions.

Main Methods:

  • Repurposing cumate, a gene expression inducer, by modifying its carboxylate group with a nitrile.
  • Utilizing nitrilase-expressing activator cells to release the caged cumate.
  • Employing reporter cells with a cumate-responsive genetic switch to detect cumate presence.

Main Results:

  • The developed system successfully illuminates cell proximity.
  • Reporter cells expressed a target gene specifically when near activator cells.
  • The strategy demonstrates a functional method for detecting cell-to-cell contact.

Conclusions:

  • This novel strategy provides a versatile platform for imaging cellular interactions.
  • The system can potentially be used to manipulate cellular interactions over time.
  • This approach offers new possibilities for studying cell proximity in biological systems.