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Related Experiment Videos

A83586C, a new depsipeptide antibiotic.

T A Smitka1, J B Deeter, A H Hunt

  • 1Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.

The Journal of Antibiotics
|June 1, 1988
PubMed
Summary

A novel cyclic hexadepsipeptide antibiotic, A83586C, was discovered from Streptomyces karnatakensis. While potent against Gram-positive bacteria in vitro, it showed no efficacy in vivo.

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Area of Science:

  • Microbiology
  • Natural Product Chemistry
  • Pharmacology

Background:

  • Streptomyces species are prolific producers of bioactive secondary metabolites.
  • Cyclic hexadepsipeptides represent a class of antibiotics with diverse biological activities.

Purpose of the Study:

  • To elucidate the structure and absolute configuration of a novel cyclic hexadepsipeptide antibiotic, A83586C.
  • To evaluate the in vitro and in vivo antimicrobial activity of A83586C.

Main Methods:

  • Structure elucidation using X-ray crystallography.
  • Nuclear Magnetic Resonance (NMR) spectroscopy for full 1H and 13C assignments.
  • Acid hydrolysis to determine absolute configuration via D-threonine detection.

Main Results:

Related Experiment Videos

  • A novel cyclic hexadepsipeptide, A83586C, was isolated and structurally characterized.
  • The absolute configuration of A83586C was confirmed.
  • A83586C exhibited potent in vitro activity against Gram-positive bacteria.

Conclusions:

  • Streptomyces karnatakensis produces a unique cyclic hexadepsipeptide antibiotic.
  • A83586C demonstrates significant in vitro antimicrobial potential.
  • Further research is needed to address the lack of in vivo efficacy.