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Switchable stimulated Raman scattering microscopy with photochromic vibrational probes.

Jianpeng Ao1, Xiaofeng Fang2, Xianchong Miao1

  • 1State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, China.

Nature Communications
|May 26, 2021
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Summary

Engineered alkyne-diarylethene probes exhibit vibrational photochromism for photo-switchable stimulated Raman scattering (SRS) imaging. This breakthrough enables reversible "on"/"off" imaging with high chemical specificity for advanced microscopy and data storage.

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Area of Science:

  • Chemical Engineering
  • Materials Science
  • Spectroscopy

Background:

  • Photochromic probes offer reversible fluorescence but lack chemical specificity.
  • Stimulated Raman Scattering (SRS) microscopy provides high chemical resolution but uses inert probes.

Purpose of the Study:

  • To develop photo-switchable probes for SRS imaging by combining photochromism and vibrational spectroscopy.
  • To engineer alkyne-tagged diarylethene molecules exhibiting vibrational photochromism.

Main Methods:

  • Synthesized alkyne-tagged diarylethene molecules.
  • Utilized ultraviolet (UV) and visible light irradiation to induce photoisomerization.
  • Performed stimulated Raman scattering (SRS) microscopy to image the vibrational changes.

Main Results:

  • Demonstrated reversible "on" and "off" SRS imaging based on the alkyne peak shift.
  • Achieved photo-rewritable patterning and encryption on thin films.
  • Successfully performed live-cell imaging, including mitochondria diffusion studies.

Conclusions:

  • Developed a novel vibrational photochromism approach for SRS imaging.
  • The alkyne-diarylethene system offers high chemical specificity and photo-switchability.
  • This design principle has potential applications in super-resolution microscopy, optical memory, and switches.