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Updated: Aug 10, 2026
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
miR‑135a‑5p inhibits tumor invasion by targeting ANGPT2 in gallbladder cancer
Haiyan Diao1, Xing Xu1, Bin Zhao1
1Department of General Surgery, The Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, P.R. China.
Abstract:
Gallbladder cancer (GBC) is the most aggressive cancer type in the biliary tract, and our previous studies observed that microRNA (miR)‑135a‑5p expression was downregulated in GBC tissues. However, few studies have focused on the mechanism of action of the miR‑135a‑5p target genes in GBC. The present study aimed to investigate the regulatory role of miR‑135a‑5p signaling in GBC. The present study found that miR‑135a‑5p expression was downregulated in GBC tissue, as detected by immunohistochemistry and reverse transcription‑quantitative PCR. In addition, overexpression of miR‑135a‑5p significantly inhibited the proliferation and migration of GBC‑SD cells. Using a luciferase activity assay, it was identified that angiopoietin‑2 (ANGPT2) was a potential target gene of miR‑135a‑5p in GBC. Knockdown of ANGPT2 expression significantly inhibited the proliferation and invasion of GBC‑SD cells. In conclusion, the present results suggested that miR‑135a‑5p affected GBC cell proliferation and invasion by targeting ANGPT2. Moreover, miR‑135a‑5p may be a potential biomarker for GBC progression and a potential target for GBC therapeutic intervention.
Insights
MicroRNA-135a-5p is downregulated in gallbladder cancer (GBC). Its restoration inhibits GBC cell proliferation and migration by targeting angiopoietin-2, suggesting potential as a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gallbladder cancer (GBC) is an aggressive biliary tract cancer.
- Previous studies indicated reduced microRNA (miR)-135a-5p expression in GBC tissues.
- The precise mechanisms of miR-135a-5p target genes in GBC remain underexplored.
Purpose of the Study:
- To investigate the regulatory role of miR-135a-5p signaling in GBC.
- To identify and validate target genes of miR-135a-5p in GBC.
- To explore the therapeutic potential of miR-135a-5p in GBC.
Main Methods:
- Immunohistochemistry and reverse transcription-quantitative PCR (RT-qPCR) to assess miR-135a-5p expression.
- GBC cell line (GBC-SD) models for overexpression and knockdown studies.
- Luciferase activity assay to identify miR-135a-5p target genes.
Main Results:
- miR-135a-5p expression was significantly downregulated in GBC tissues.
- Overexpression of miR-135a-5p suppressed GBC cell proliferation and migration.
- Angiopoietin-2 (ANGPT2) was identified as a direct target gene of miR-135a-5p.
- Knockdown of ANGPT2 inhibited GBC cell proliferation and invasion.
Conclusions:
- miR-135a-5p inhibits GBC cell proliferation and invasion by targeting ANGPT2.
- miR-135a-5p demonstrates potential as a biomarker for GBC progression.
- miR-135a-5p represents a promising therapeutic target for GBC intervention.
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