Clot characterization by multidisciplinary approach: biochemical and imaging parameters in a hypocoagulative setting....

Area of Science:

• Hematology
• Biophysics
• Medical Imaging

Background:

• Clot characterization currently uses multimodal approaches including SEM, CWA, and thrombin generation.
• Existing methods provide insights into fibrin structure and laboratory parameters of clot abnormalities.

Purpose of the Study:

• To assess the consistency between coagulative parameters and SEM-acquired clot image textures.
• To propose an integrative approach for clot classification.

Main Methods:

• Pilot study included healthy subjects, Acquired Hemophilia A (AHA) patients, and Vitamin K Antagonist (VKA) patients.
• Evaluated clot formation velocity/acceleration (CWA), thrombin generation, and SEM clot imaging.
• Analyzed SEM images using MATLAB texture analysis (Contrast, Energy) for classification.

Main Results:

• Significant differences in coagulative parameters were found among healthy subjects, AHA, and VKA groups.
• SEM texture parameters (Contrast, Energy) showed significant differences aligning with laboratory findings.
• No significant differences were detected between VKA and AHA patient groups for either method.

Conclusions:

• SEM, CWA, and thrombin generation parameters offer a multiparametric starting point for clot formation studies.
• This integrated approach aids in characterizing clot formation across diverse clinical conditions.