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Related Concept Videos

Biofilms01:29

Biofilms

574
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
574

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Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
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Biomaterial and Biofilm Interactions with the Pulp-Dentin Complex-on-a-Chip.

N S Rodrigues1, C M França2, A Tahayeri2

  • 1Post-Graduation Program in Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.

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|May 26, 2021
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Summary

Calcium silicate cements (CSCs) promote pulp repair, but early interactions are unclear. A "tooth-on-a-chip" model showed ProRoot and Biodentine enhanced cell proliferation, while TheraCal reduced viability, and ProRoot demonstrated antimicrobial effects.

Keywords:
bioassayscalcium silicate cementsgrowth factorsmicrofluidicsorgans-on-a-chipstem cells

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Area of Science:

  • Biomaterials Science
  • Dental Research
  • Tissue Engineering

Background:

  • Calcium silicate cements (CSCs) are vital for pulp repair due to bioactivity and dentin bridge formation.
  • Early interactions between CSCs, dentin, and pulp are not fully understood, hindering optimal material selection.
  • Existing research lacks models that accurately replicate the in vivo dentin-pulp complex environment.

Purpose of the Study:

  • To investigate the early biological responses of human dental pulp stem cells (hDPSCs) to different CSCs (ProRoot, Biodentine, TheraCal).
  • To analyze pH variations and transforming growth factor-β (TGFβ) release from CSCs within a simulated pulp chamber.
  • To evaluate the real-time antimicrobial efficacy of CSCs against *Streptococcus mutans* biofilms.

Main Methods:

  • Development and utilization of a microfluidic
  • tooth-on-a-chip
  • device to mimic the biomaterial-dentin-pulp interface.
  • Assessment of hDPSCs viability, proliferation, and morphology upon exposure to ProRoot, Biodentine, and TheraCal.
  • Measurement of TGFβ release and pH changes in the simulated pulp environment.
  • Real-time live/dead assay of *Streptococcus mutans* biofilms interacting with ProRoot.

Main Results:

  • ProRoot significantly increased TGFβ extraction within 24-72 hours (P < 0.05).
  • ProRoot and Biodentine enhanced hDPSCs proliferation (P > 0.05), while TheraCal decreased viability and altered cell morphology.
  • No correlation was found between TGFβ levels and pH variations.
  • ProRoot demonstrated significant real-time antimicrobial activity against *S. mutans* biofilms.

Conclusions:

  • The
  • tooth-on-a-chip
  • model effectively characterizes early interactions between bioactive dental materials and the dentin-pulp complex.
  • ProRoot and Biodentine show promising biocompatibility and bioactivity for vital pulp therapy, promoting cell proliferation and TGFβ release.
  • TheraCal exhibited cytotoxic effects, indicating potential limitations for direct pulp capping applications.
  • The model enables real-time assessment of antibiofilm properties, crucial for evaluating material efficacy in infected environments.