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Related Concept Videos

RNA Structure01:23

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Related Experiment Video

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BRIO: a web server for RNA sequence and structure motif scan.

Andrea Guarracino1, Gerardo Pepe1, Francesco Ballesio1

  • 1Department of Biology, University of Rome "Tor Vergata", Rome, Italy.

Nucleic Acids Research
|May 26, 2021
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Summary

Researchers developed BRIO, a web server to identify RNA-binding motifs. This tool helps understand how RNA-binding proteins (RBPs) regulate gene expression and other cellular processes.

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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • RNA-binding proteins (RBPs) are crucial for gene expression regulation.
  • RBPs bind specific RNA sequence and structure motifs to perform their functions.
  • Understanding these motifs is key to deciphering cellular processes.

Purpose of the Study:

  • To introduce BRIO, a novel web server for identifying RNA-binding motifs.
  • To facilitate the discovery of sequence and structure motifs associated with RBP binding.

Main Methods:

  • Development of the BRIO web server.
  • Integration of a comprehensive database of RNA-binding motifs.
  • Utilizing data from PAR-CLIP, eCLIP, and HITS experiments.

Main Results:

  • BRIO provides access to 2508 sequence and 2296 structure motifs.
  • The motifs are linked to 186 RBPs and 69 protein domains in humans and mice.
  • The web server is freely available for public use.

Conclusions:

  • BRIO is a valuable resource for researchers studying RNA-protein interactions.
  • The tool enhances the understanding of gene regulation by RBPs.
  • Facilitates motif identification for improved knowledge of cellular mechanisms.