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Updated: Nov 4, 2025

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
Published on: November 11, 2022
Noninvasive Laser Microtomography of a Human Isolated Oocyte
A G Pogorelov1, N P Makarova2, A P Sysoeva2
1Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia. agpogorelov@rambler.ru.
Abstract:
Using a non-invasive approach, quantitative laser scanning microtomography (QLSM), the morphology of human oocyte was studied layer-by-layer. Then, the cell volume was computed based on 3D reconstruction of a stack of optical sections obtained by QLSM. The integrity of oocyte membrane after cryopreservation was assessed by measuring the changes in oocyte volume in response to hypotonic shock.
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