Targeting Jab1 using hesperidin (dietary phytocompound) for inducing apoptosis in HeLa cervical cancer cells
Pratibha Pandey1, Fahad Khan1, Pooja Maurya1
1Department of Biotechnology, Noida Institute of Engineering and Technology, Greater Noida, India.
Abstract:
Plant flavonoids have been emerged as a potent anticancerous agent by exhibiting significant growth inhibitory potential and apoptotic induction in several carcinomas via targeting several oncoproteins. However, inverse association of hesperidin with Jab1 oncoprotein in cervical cancer has rarely been reported. Thus, we have intended our research study towards establishing this unexplored inverse correlation of hesperidin with Jab1 which could further prevent cervical cancer progression. Our research findings clearly demonstrated that hesperidin treatment resulted in Jab1 gene down-regulation and p27 up-regulation in a dose-dependent manner in HeLa cancer cells. These gene modulations might occur via excessive reactive oxygen species (ROS) generation and caspase-3 activation which further resulted in apoptotic induction. Increase in apoptotic cells was confirmed through Hoechst staining and cell cycle analysis. Thus, these results strongly suggested that Jab1 is a potent therapeutic target of hesperidin to suppress cell growth and trigger apoptosis in HeLa cells. PRACTICAL APPLICATIONS: Dietary flavonoids play a crucial role in the management of numerous malignancies via targeting several mutated oncogenes. Our study strongly exhibited that hesperidin treatment suppressed the HeLa cancer cell proliferation via increased ROS generation and reduced Jab1 mRNA expression. Thus, the inference of Jab1-mediated intracellular signals by hesperidin might be a novel approach to control cervical cancer.
Insights
Hesperidin, a plant flavonoid, suppresses cervical cancer growth by down-regulating Jab1 oncoprotein and inducing apoptosis in HeLa cells. This study highlights hesperidin
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Plant flavonoids show anticancer potential by inhibiting tumor growth and inducing apoptosis.
- Hesperidin's inverse association with Jab1 oncoprotein in cervical cancer is largely unexplored.
- Targeting oncoproteins is a key strategy in cancer management.
Purpose of the Study:
- To investigate the inverse correlation between hesperidin and Jab1 oncoprotein in cervical cancer.
- To establish hesperidin as a potential therapeutic agent for cervical cancer progression.
- To elucidate the molecular mechanisms underlying hesperidin's anticancer effects.
Main Methods:
- HeLa cancer cells were treated with varying doses of hesperidin.
- Gene expression analysis (Jab1, p27) and reactive oxygen species (ROS) generation were measured.
- Caspase-3 activation, Hoechst staining, and cell cycle analysis were performed to assess apoptosis.
Main Results:
- Hesperidin treatment led to dose-dependent down-regulation of Jab1 and up-regulation of p27 in HeLa cells.
- Increased ROS generation and caspase-3 activation were observed following hesperidin treatment.
- Apoptosis induction was confirmed by Hoechst staining and cell cycle analysis, indicating suppressed cell proliferation.
Conclusions:
- Jab1 is a potent therapeutic target of hesperidin for suppressing cell growth and inducing apoptosis in HeLa cells.
- Hesperidin's mechanism involves ROS generation and caspase-3 activation, leading to apoptosis.
- Targeting Jab1-mediated signals by hesperidin offers a novel approach for cervical cancer control.
