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Updated: Nov 4, 2025

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Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
Published on: October 6, 2022
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Lipid Isolation from Plants
Jesse D Bengtsson1, James G Wallis1, John Browse2
1Institute of Biological Chemistry, Washington State University, Pullman, WA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 28, 2021
Abstract:
Analysis of plant lipids provides insights into a range of biological processes, from photosynthetic membrane function to oil seed engineering. Many lipid extraction protocols are tailored to fit a specific lipid class. Here we describe a procedure for extraction of glycerolipids from vegetative tissue. This procedure is designed for 1 gram of tissue per sample but maybe scaled for larger samples.

