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Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging.
Bryce T Bajar1, Michael Z Lin2,3
1Department of Biological Chemistry, Medical Scientist Training Program, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 29, 2021
Summary
This study presents Fucci4, a fluorescent biosensor for visualizing cell cycle progression in live cells. This method allows dynamic tracking of cell cycle transitions using epifluorescence microscopy.
Area of Science:
- Cell biology
- Molecular biology
- Biophysics
Background:
- Cell cycle progression is crucial for understanding development, tissue homeostasis, and diseases like cancer.
- Fluorescent biosensors enable dynamic monitoring of molecular events, including cell cycle dynamics.
- Tracking cell cycle phases is essential for research in developmental biology and oncology.
Purpose of the Study:
- To describe a method for tracking cell cycle progression using the Fucci4 fluorescent biosensor.
- To enable visualization of transitions between cell cycle phases in live cells.
- To provide a adaptable imaging technique for studying cell proliferation.
Main Methods:
- Utilizing the genetically encoded Fucci4 fluorescent indicator system.
- Employing live epifluorescence microscopy for dynamic cell tracking.
- Adapting in vitro time-lapse imaging for four spectrally distinct fluorescent indicators.
Main Results:
- Demonstrated successful visualization of cell cycle phase transitions using Fucci4.
- Established a reliable method for tracking cell cycle progression in real-time.
- Validated the adaptability of the technique for various fluorescent reporters.
Conclusions:
- Fucci4 provides a powerful tool for visualizing cell cycle dynamics.
- The described epifluorescence imaging method offers a versatile approach for cell cycle studies.
- This technique can be applied to diverse research areas, including cancer biology and developmental studies.
