pH-Responsive Capsules with a Fibril Scaffold Shell Assembled from an Amyloidogenic Peptide.

Area of Science:

• Biomaterials Science
• Supramolecular Chemistry
• Nanotechnology

Background:

• Peptides and proteins self-assemble into functional supramolecular structures via non-covalent interactions.
• Biomolecular assembly is sensitive to environmental factors like pH, temperature, and light.
• This sensitivity inspires the creation of responsive functional materials from peptide building blocks.

Purpose of the Study:

• To demonstrate the use of stimuli-responsive assembly of amyloidogenic peptides for creating environmentally responsive microcapsules.
• To investigate pH-triggered release characteristics of these peptide-based microcapsules.
• To explore their potential as vehicles for controlled release of biomolecules.

Main Methods:

• Utilized amyloidogenic peptides for self-assembly into microcapsule structures.
• Employed pH modulation to trigger and control the assembly and disassembly of microcapsules.
• Analyzed microcapsule structure using Cryo-Scanning Electron Microscopy (Cryo-SEM).
• Assessed the encapsulation and release of cargo molecules, including antibodies.

Main Results:

• Developed biocompatible and biodegradable microcapsules with pH-triggered release capabilities.
• Cryo-SEM revealed an internal fibrillar network forming compartments for cargo storage.
• Demonstrated reversible microcapsule formation by adjusting solution pH.
• Showcased potential for controlled release of encapsulated biomolecules like antibodies.

Conclusions:

• Peptide-based microcapsules offer a promising platform for pH-responsive molecular encapsulation.
• These materials can serve as vehicles for controlled storage and release of diverse biomolecules.
• The approach provides a versatile strategy for developing functional peptide-based materials.