Related Experiment Video

Updated: Nov 4, 2025

Author Spotlight: Investigating Bacteriophage-Induced Immune Responses in Gnotobiotic Mice
08:46

Author Spotlight: Investigating Bacteriophage-Induced Immune Responses in Gnotobiotic Mice

Published on: January 26, 2024

2.1K

Characterization of a novel group I F-specific RNA bacteriophage isolated from human stool

Mingshu Yang1, Yongxin Yu1,2, Yongjie Wang3,4,5

  • 1College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.

Archives of Microbiology
|May 29, 2021
PubMed

Insights

A novel F-specific RNA bacteriophage (FRNAPH) named YM1 was isolated from human stool. This phage shows high specificity for Escherichia coli and could be a valuable indicator for environmental enteric virus contamination.

Area of Science:

  • Virology
  • Microbiology
  • Environmental Science

Background:

  • F-specific RNA bacteriophages (FRNAPHs) are common in the environment.
  • Levivirus genus includes important FRNAPHs.
  • Human stool is a potential source for novel phage discovery.

Purpose of the Study:

  • To isolate and characterize a novel FRNAPH from human stool.
  • To determine the genomic and biological properties of the new phage.
  • To evaluate its potential as a viral indicator.

Main Methods:

  • Isolation using double-layer agar plates with Escherichia coli ATCC700891.
  • Whole-genome sequencing using next-generation sequencing.
  • Morphological characterization and one-step growth curve analysis.

Main Results:

  • A novel FRNAPH, YM1, was isolated and identified as genogroup I (GI) Levivirus.
  • The complete genome is 3551 nt, encoding four proteins: maturation, coat, lysis, and RdRp.
  • YM1 shares 95.3% genomic similarity with GI FRNAPH DL16 and exhibits high host specificity for E. coli.

Conclusions:

  • YM1 is a novel GI Levivirus isolated from human feces.
  • Its characteristics suggest potential application as a viral indicator for enteric viruses like norovirus.
Keywords:
F-specific RNA bacteriophageGroup IHuman stool

More Related Videos

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

11.2K
Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

1.3K

Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
343
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
73.8K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
612
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
293
Bacterial Phylum Bacteroidota01:26

Bacterial Phylum Bacteroidota

The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
244
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
64.7K